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Western blot analysis of RHBG Antibody in K562 cell line lysates (35ug/lane)
RHBG Antibody IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of RHBG Antibody in K562 cell line lysates (35ug/lane)

RHBG Antibody

CATALOG NUMBER: 64-221

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RHBG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-450 amino acids from the C-terminal region of human RHBG.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RHBG
Additional Names:
Ammonium transporter Rh type B, Rhesus blood group family type B glycoprotein, Rh family type B glycoprotein, Rh type B glycoprotein, RHBG
Protein Accession Number:
Q9H310
Protein Gi Number:
209572666
Ncbi Gene Id Number:
57127
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RHBG encodes one of two non-erythroid members of the Rhesus (Rh) protein family. Non-erythroid Rh protein family members are mainly expressed in the kidney and belong to the methylammonium-ammonium permease/ammonia transporters superfamily. All Rh family proteins are predicted to be transmembrane proteins with 12 membrane spanning domains and intracytoplasmic N- and C-termini.
Background Reference 1:
Han, K.H., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 297 (1), L153-L163 (2009)
Background Reference 2:
Brown, A.C., et al. Am. J. Physiol. Renal Physiol. 296 (6), F1279-F1290 (2009)
Background Reference 3:
Sohet, F., et al. J. Biol. Chem. 283(39):26557-26567(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-221

List Size:
400 ul

List Price:

$495.00

Datasheet
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