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Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Human B7-2, Fc Tag with a linear range of 0.313-5 ug/mL (QC tested).
Immobilized Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Human B7-1, Fc Tag with a linear range of 0.625-10 ug/mL (Routinely tested).
Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Rhesus macaque CD28 Recombinant Protein

CATALOG NUMBER: 11-530

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human, Cynomolgus, Rhesus macaque
source-species:
HEK293 cells
Recombinant Protein Sequence:
Asn 19 - Pro 152
Fusion Tag:
Mouse IgG Fc Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 42.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted Molecular Weight:
42.0 kDa
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
50 mM Tris, 100 mM Glycine, pH7.5
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CD28
Additional Names:
CD28,Tp44
Protein Accession Number:
NP_006130.1
Ncbi Gene Id Number:
940
Background:
T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Background Reference 1:
Evans E.J., et al., 2005, Nat. Immunol. 6:271-279.
Background Reference 2:
Mayya V., et al., 2009, Sci. Signal. 2:RA46-RA46.
Background Reference 3:
Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-530

List Size:
0.1 mg

List Price:

$515.00

Datasheet
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