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RHOJ Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HepG2,MDA-MB231 cell line lysates (35ug/lane).
RHOJ Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

RHOJ Antibody

CATALOG NUMBER: 56-029

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RHOJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-159 amino acids from the Central region of human RHOJ.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RHOJ
Additional Names:
Rho-related GTP-binding protein RhoJ, Ras-like protein family member 7B, Tc10-like GTP-binding protein, RHOJ, ARHJ, RASL7B, RHOI, TCL
Protein Accession Number:
Q9H4E5
Protein Gi Number:
24418646
Ncbi Gene Id Number:
57381
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ARHJ belongs to the Rho family of small GTP-bindingproteins. Rho proteins regulate the dynamic assembly ofcytoskeletal components for several physiologic processes, such ascell proliferation and motility and the establishment of cellpolarity. They are also involved in pathophysiologic process, suchas cell transformation and metastasis.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Background Reference 3:
Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005)
Background Reference 4:
Nishikimi, A., et al. FEBS Lett. 579(5):1039-1046(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-029

List Size:
400 ul

List Price:

$495.00

Datasheet
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