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Western blot analysis of RHOT1 in rat brain tissue lysate with RHOT1 antibody at 1 μg/ml.
Immunohistochemistry of RHOT in mouse brain tissue with RHOT antibody at 5 μg/ml.
Immunofluorescence of RHOT in mouse brain tissue with RHOT antibody at 20 μg/ml.
Western blot analysis of RHOT1 in rat brain tissue lysate with RHOT1 antibody at 1 μg/ml.
Trial Size

RHOT1 Antibody

CATALOG NUMBER: 8027

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (81%)
Immunogen:
RHOT1 antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOT1.
The immunogen is located within amino acids 40 - 90 of RHOT1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
RHOT1 antibody is human, mouse and rat reactive. At least three isoforms of RHOT1 are known to exist; this antibody will detect all three isoforms.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 76 kDa
Observed: 78 kDa
Purification:
RHOT1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RHOT1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RHOT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
RHOT1
Additional Names:
Ras homolog family member T1, mitochondrial Rho GTPase 1, ARHT1, MIRO-1, MIRO1, MIRO
Protein Accession Number:
NP_001028740
Protein Gi Number:
75750480
Ncbi Gene Id Number:
55288
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1). RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria (2,3). Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4).
Background Reference 1:
Fransson A, Ruusala A, and Aspenstrom P. Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis. J. Biol. Chem. 2003; 278:6495-502.
Background Reference 2:
Macaskill AF, Brickley K, Stephenson FA, et al. GTPase dependent recruitment of Grif-1 by Miro1 regulates mitochondrial trafficking in hippocampal neurons. Mol. Cell. Neurosci. 2009;40:301–12.
Background Reference 3:
Weihofen A, Thomas KJ, Ostaszewski B, et al. Pink1 forms a multi-protein complex with Miro and Milton, linking Pink1 function to mitochondrial trafficking. Biochemistry 2009; 48:2045-54.
Background Reference 4:
Wang X, Winter D, Ashrafi G, et al. PINK1 and Parkin target Miro for phosphorylation and degradation to arrest mitochondrial motility. Cell 2011; 147:893-906.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8027

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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