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43-054 (1ug/ml) staining of HeLa and Mouse D3 (ES) lysates (35ug protein in RIPA buffer). Data obtained from Hisao Masai, Tokyo Metropolitan Institure of Medical Science, Japan. Primary incubation was 1 hour. Detected by chemiluminescence. <strong>This d
43-054 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blu
43-054 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)
43-054 (1ug/ml) staining of HeLa and Mouse D3 (ES) lysates (35ug protein in RIPA buffer). Data obtained from Hisao Masai, Tokyo Metropolitan Institure of Medical Science, Japan. Primary incubation was 1 hour. Detected by chemiluminescence. This d

RIF1 Antibody

CATALOG NUMBER: 43-054

Clonality:
Polyclonal
Tested Applications:
ELISA, IF
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-RNLQSRWRSPSHEN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3 and U2OS cells. Recommended concentration: 10ug/ml
Specificity:
This antibody is expected to recognize both reported isoforms (NP_060621.3; NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.1, NP_001171134.1
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
Approx 270 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RIF1
Additional Names:
RIF1, RAP1 interacting factor homolog (yeast), DKFZp434D1026, DKFZp781N1478, FLJ12870, OTTHUMP00000162712, OTTHUMP00000207206, OTTHUMP00000207207, OTTHUMP00000207208, OTTHUMP00000207210, rap1-interacting factor 1 homolog, telomere-associated protein RIF1
Protein Accession Number:
NP_060621.3,NP_001171134.1
Protein Gi Number:
56676335
Ncbi Gene Id Number:
55183

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-054

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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