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46-300 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-300 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-300 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

RIF1 Antibody

CATALOG NUMBER: 46-300

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CEEGIIDANKTETNT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 247kDa according to NP_060621. Therefore we cannot recommend an optimal concentration.Immunohistochemistry:Paraffin embedded Human Testis and Brain (Cortex). Recommended concentration: 1-2ug/ml.
Specificity:
This antibody is expected to recognise an epitope corresponding to aa 2076-2089 of human RIF1 protein.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RIF1
Additional Names:
Rif1, telomere-associated protein RIF1 homolog, Rap1 interacting factor 1, RAP1 interacting factor homolog (yeast), DKFZp434D1026, DKFZp781N1478, FLJ12870, OTTHUMP00000162712, RAP1 interacting factor 1
Protein Accession Number:
NP_060621.3
Protein Gi Number:
56676335
Ncbi Gene Id Number:
55183
Background Reference 1:
Silverman J, Takai H, Buonomo SB, Eisenhaber F, de Lange T. Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. Genes Dev. 2004 Sep 1;18(17):2108-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-300

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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