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Western blot analysis of various lysates, using Rig-I/DDX58 Rabbit mAb at 1:1000 dilution. THP-1 cells were treated with LPS (1 μg/ml) at 37℃ for 8 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.
Western blot analysis of various lysates, using Rig-I/DDX58 Rabbit mAb at 1:1000 dilution. THP-1 cells were treated with LPS (1 μg/ml) at 37℃ for 8 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.

Rig-I/DDX58 Rabbit Recombinant Antibody (83-741)

CATALOG NUMBER: 83-741

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
U-87MG,THP-1 treated with LPS
Predicted Molecular Weight:
107kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RIGI
Additional Names:
RIG1; DDX58; RIG-I; RLR-1; SGMRT2; Rig-I/DDX58
Protein Accession Number:
O95786
Ncbi Gene Id Number:
23586
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-741

List Size:
100 uL

List Price:

$477.00

Datasheet
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