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RILPL1 Antibody

CATALOG NUMBER: 6495

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
RILPL1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human RILPL1.
The immunogen is located within the last 50 amino acids of RILPL1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
RILPL1 antibody can be used for detection of RILPL1 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of RILPL1 are known to exist; this antibody will detect the two shorter isoforms. RILPL1 antibody is predicted to not cross-react with RILP.
Positive Control 1:
Cat. No. 1473 - Rat Cerebellum Tissue Lysate
Purification:
RILPL1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RILPL1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RILPL1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RILPL1
Additional Names:
RILPL1 Antibody: RLP1, GOSPEL, RLP1, RILP-like protein 1, Rab-interacting lysosomal-like protein 1
Protein Accession Number:
AAH89444
Protein Gi Number:
195947382
Ncbi Gene Id Number:
353116
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RILPL1 Antibody: While the function of the Rab interacting lysosomal protein-like 1 (RILPL1) remains unknown, the homologous protein RILP is a small GTPase that controls transport to endocytic compartments, suggesting RILPL1 may have similar functions. However, overexpression of RILP caused enlarged lysosomes that are more centrally located in the cell, while overexpression of RILPL1 had no visible effect on the location or morphology of lysosomes.
Background Reference 1:
Cantalupo G, Alifano P, Roberti V, et al. Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes. EMBO J. 2001; 20:683-93
Background Reference 2:
Wang T, Wong KK, and Hong W. A unique region of RILP distinguishes it from its related proteins in its regulation of lysosomal morphology and interaction with Rab7 and Rab34. Mol. Biol. Cell 2004; 15:815-26

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6495

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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