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Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).

RING1 Antibody

CATALOG NUMBER: 57-819

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This RING1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-123 amino acids from the N-terminal region of human RING1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RING1
Additional Names:
E3 ubiquitin-protein ligase RING1, 632-, Polycomb complex protein RING1, RING finger protein 1, Really interesting new gene 1 protein, RING1, RNF1
Protein Accession Number:
Q06587
Protein Gi Number:
90110053
Ncbi Gene Id Number:
6015
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene belongs to the RING finger family, members ofwhich encode proteins characterized by a RING domain, azinc-binding motif related to the zinc finger domain. The geneproduct can bind DNA and can act as a transcriptional repressor. Itis associated with the multimeric polycomb group protein complex.The gene product interacts with the polycomb group proteins BMI1,EDR1, and CBX4, and colocalizes with these proteins in largenuclear domains. It interacts with the CBX4 protein via itsglycine-rich C-terminal domain. The gene maps to the HLA class IIregion, where it is contiguous with the RING finger genes FABGL andHKE4.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
de Bie, P., et al. Biochem. Biophys. Res. Commun. 400(3):389-395(2010)
Background Reference 3:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Background Reference 4:
Barcellos, L.F., et al. PLoS Genet.(10), E1000696 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-819

List Size:
400 ul

List Price:

$495.00

Datasheet
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