RIP3 Antibody [Rippy-3]

CATALOG NUMBER: XA-1023

Clonality:
Monoclonal
Tested Applications:
WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
RIP3 (monoclonal Rippy-3) antibody was raised against recombinant human RIP3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
RIP3 antibody can be used to recognize human RIP3 by western blot. Method: HEK 293T cells (5x105) mock transfected (lane 1) or transfected (lane 2) with an expression plasmid coding for human RIP3 were harvested, lysed and cell extracts (5x105) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the Rippy-3 monoclonal antibody at a 1:1000 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system. This product is for research use only.
Purification:
RIP3 (monoclonal Rippy-3) antibody was purified to >=95% in SDS-PAGE.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Storage Conditions:
RIP3 antibody can be stored at 4°C, stable for one year.
Ncbi Gene Id Number:
11035
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background Reference 1:
Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB: P.W. Yu, et al.; Curr. Biol. 9, 539 (1999).
Background Reference 2:
RIP3, a novel apoptosis-inducing kinase: X. Sun, et al.; J. Biol. Chem. 274, 16871 (1999).
Background Reference 3:
Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB: N.J. Pazdernik, et al.; Mol. Cell. Biol. 19, 6500 (1999).
Background Reference 4:
RIP4 (DIK/PKK), a novel member of the RIP kinase family, activates NF-kappa B and is processed during apoptosis: E. Meylan, et al.; EMBO Rep. 3, 1201 (2002).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

XA-1023

List Size:
0.1 mg

List Price:

$546.00

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