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<strong> Figure 1 WB Validation in Human, Mouse and Rat Cells </strong><br>Loading: 15 μg of lysate Antibodies: RIPK1, 5389,2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 WB Validation in Mouse Tissues</strong><br>Loading: 15 μg of lysate Antibodies: RIPK1, 5389, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong> Figure 3 WB Validation in Rat Tissues </strong><br>Loading: 15 μg of lysate Antibodies: RIPK1, 5389, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of RIPK1 in Human Liver</strong><br> Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RIPK1 antibody (5389) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunohistochemistry Validation of RIPK1 in Mouse Colon</strong><br> Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RIPK1 antibody (5389) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunohistochemistry Validation of RIPK1 in Rat Colon</strong><br> Immunohistochemical analysis of paraffin-embedded rat colon tissue using anti-RIPK1 antibody (5389) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of RIPK1 in Mouse Kidney</strong><br> Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-RIPK1 antibody (5389) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 WB Validation in Human, Mouse and Rat Cells
Loading: 15 μg of lysate Antibodies: RIPK1, 5389,2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

RIPK1 Antibody

CATALOG NUMBER: 5389

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-RIPK1 antibody (5389) was raised against a peptide corresponding to 15 amino acid synthetic peptide near the amino terminus of human RIPK1.

The immunogen is located within amino acids 180-230 of RIPK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB: 1-4 μg/mL; IHC-P: 1-2.5 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1465 - Rat Kidney Tissue Lysate
Positive Control 2:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 3:
Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 76 kD

Observed: 80 kD
Purification:
RIPK1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RIPK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RIPK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RIPK1
Additional Names:
RIPK1 Antibody: RIP, RIP1, RIP, Cell death protein RIP, RIP-1
Protein Accession Number:
NP_003795
Protein Gi Number:
57242761
Ncbi Gene Id Number:
8737
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RIPK1 Antibody: RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NFκ-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-κB activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.
Background Reference 1:
Stanger BZ, Leder P, Lee TH, et al. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell1995; 81:513-23.
Background Reference 2:
Hsu H, Huang J, Shu HB, et al. TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex. Immunity1996; 4:387-96.
Background Reference 3:
Meylan E, Burns K, Hofmann K, et al. RIP1 is an essential mediator of Toll-like receptor 3-induced NF-kappa B activation. Nat. Immunol.2004; 5:503-7.
Background Reference 4:
Festjens N, Vanden Bergh T, Cornelis S, et al. RIP1, a kinase on the crossroads of a cell's decision to live or die. Cell Death Differ.2007;14:400-10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5389

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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