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Antibody used in IHC on Human Liver cell lysates.
Antibody used in IHC on Human Heart lysate.
Antibody used in WB on Human Jurkat 1.25 ug/ml.
Antibody used in IHC on Human Liver cell lysates.

RIPK3 Antibody

CATALOG NUMBER: 27-361

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RIPK3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
RIPK3 antibody can be used for detection of RIPK3 by ELISA at 1:62500. RIPK3 antibody can be used for detection of RIPK3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
57 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store RIPK3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
RIPK3
Additional Names:
RIPK3, RIP3, RIP3 beta, RIP3 gamma
Protein Accession Number:
NP_006862
Protein Gi Number:
40254844
Ncbi Gene Id Number:
11035
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.
Background Reference 1:
Kimura, K., (2006) Genome Res. 16 (1), 55-65.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-361

List Size:
100 ul

List Price:

$473.00

Datasheet
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