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Western blot analysis of RKHD3 in human testis tissue lysate with RKHD3 antibody at 1 μg/mL.
Immunofluorescence of RKHD3 in mouse skeletal muscle cells with RKHD3 antibody at 20 μg/mL.
Western blot analysis of RKHD3 in human testis tissue lysate with RKHD3 antibody at 1 μg/mL.
Trial Size

RKHD3 Antibody

CATALOG NUMBER: 6641

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
RKHD3 antibody was raised against a 16 amino acid synthetic peptide from near the center of human RKHD3.
The immunogen is located within amino acids 180 - 230 of RKHD3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
RKHD3 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
This antibody is specific for RKHD3 and will not recognize the other RKHD3 family of proteins.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight:
Predicted: 62 kDa
Observed: 62 kDa
Purification:
RKHD3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RKHD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RKHD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MEX3B
Additional Names:
RKHD3 Antibody: RKHD3, MEX-3B, RNF195, KIAA2009, RKHD3, RNA-binding protein MEX3B, RING finger and KH domain-containing protein 3
Protein Accession Number:
NP_780575
Protein Gi Number:
47716512
Ncbi Gene Id Number:
84206
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RKHD3 Antibody: Rkhd3, also known as MEX3B is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd3, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. Rkhd3 is thought to act as a mechanism to fine-tune mRNA regulation in early Xenopus development, and with Rkhd4, but not Rkhd1, will co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.
Background Reference 1:
Takada H, Kawana T, Ito Y, et al. The RNA-binding protein Mex3b has a fine-tuning system for mRNA regulation in early Xenopus development. Dev. 2009; 136:2413-22
Background Reference 2:
Draper BW, Mello CC, Bowerman B, et al. MEX-3 is a KH domain protein that regulates blastomere identity in early C. elegans embryos. Cell 1996; 87:205-16.
Background Reference 3:
Liu J, Valencia-Sanchez MA, Hannon GJ, et al. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat. Cell Biol 2005; 7:719-23.
Background Reference 4:
Buchet-Poyau K, Courchet J, Le Hir H, et al. Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies. Nucleic Acids Res. 2007; 35:1289-300.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6641

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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