Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of RLN3 in HeLa cell lysate with RLN3 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of RLN3 in human brain tissue with RLN3 antibody at 5 μg/mL.
Immunofluorescence of RLN3 in human brain tissue with RLN3 antibody at 20 μg/mL.
Western blot analysis of RLN3 in HeLa cell lysate with RLN3 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

RLN3 Antibody

CATALOG NUMBER: 8181

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (89%), Rat: (84%)
Immunogen:
RLN3 antibody was raised against a 19 amino acid peptide near the center of human RLN3.
The immunogen is located within amino acids 80 - 130 of RLN3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RLN3 antibody can be used for detection of RLN3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
RLN3 antibody is human specific. RLN3 antibody is predicted to not cross-react with other members of the Relaxin protein family.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 16 kDa
Observed: 16 kDa
Purification:
RLN3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RLN3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RLN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
RLN3
Additional Names:
Relaxin-3, RXN3, H3, ZINS4, INSL7
Protein Accession Number:
NP_543140
Protein Gi Number:
18254464
Ncbi Gene Id Number:
117579
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Relaxin-3 (RLN3) is a member of Relaxin family, a group of endocrine and autocrine/paracrine hormones that belong to the insulin gene superfamily (1,2). RLN3 can bind to and activate three relaxin-family peptide receptors: RXFP1, RXFP3, and RXFP4 (3). In contrast to the widespread expression of the related proteins RLN1 and RLN2, RLN3 is predominantly expressed in brain and to a lesser extent in the thymus, kidney and spleen (1). RLN3 has been suggested to play a role in feeding, metabolism, stress, arousal, learning, and memory (4).
Background Reference 1:
Bathgate RA, Samuel CS, Burazin TC, et al. Human relaxin gene 3 (H3) and the equivalent mouse relaxin (M3) gene. Novel members of the relaxin peptide family. J. Biol. Chem. 2002; 277:1148-57.
Background Reference 2:
Wilkinson TN and Bathgate RA. The evolution of the relaxin peptide family and their receptors. Adv. Exp. Med. Biol. 2007; 612:1-13.
Background Reference 3:
Silvertown JD, Neschadim A, Liu HN, et al. Relaxin-3 and receptors in the human and rhesus brain and reproductive tissues. Regul. Pept. 2010; 159:44-53.
Background Reference 4:
Bathgate RAD, Halls ML, van der Westhuizen ET, et al. Relaxin family peptides and their receptors. Physiol. Rev. 2012; 93:405-80.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8181

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369