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Western blot analysis of RLP2 in A549 cell lysate with RLP2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of RLP2 in human lung tissue with RLP2 antibody at 2.5 μg/mL.
Immunofluorescence of RLP2 in human lung tissue with RLP2 antibody at 20 μg/mL.
Western blot analysis of RLP2 in A549 cell lysate with RLP2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

RLP2 Antibody

CATALOG NUMBER: 6799

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%), Rat: (100%), Bovine: (100%)
Immunogen:
RLP2 antibody was raised against an 16 amino acid synthetic peptide near the center of human RLP2.
The immunogen is located within amino acids 110 - 160 of RLP2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RLP2 antibody can be used for detection of RLP2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Purification:
RLP2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RLP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RLP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RILPL2
Additional Names:
RLP2 Antibody: RLP2, RLP2, RILP-like protein 2, Rab-interacting lysosomal protein-like 2
Protein Accession Number:
NP_659495
Protein Gi Number:
21450828
Ncbi Gene Id Number:
196383
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RLP2 Antibody: The Rab interacting lysosomal protein-like 2 (RLP2) protein is related to the is lysosomal protein RILP which interacts with RAB7, a small GTPase that controls transport to endocytic degradative compartments. Little is known of the exact function of RLP2, but it is thought that RILP is a downstream effector of RAB7 and both proteins act together in the regulation of late endocytic traffic, suggesting that RLP2 may be involved in endocytic traffic with RILP or play a similar role. RILP and possible RLP2 may also play a role in the biogenesis of multivesicular bodies.
Background Reference 1:
Wang T, Wong KK, and Hong W. A unique region of RILP distinguishes it from its related proteins in its regulation of lysosomal morphology and interaction with Rab7 and Rab34. Mol. Biol. Cell 2004; 15:815-26.
Background Reference 2:
Cantalupo G, Alifano P, Roberti V, et al. Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes. EMBO J. 2001; 20:683-93.
Background Reference 3:
Progida C, Spinosa MR, De Luca A, et al. RILP interacts with the VPS22 component of the ESCRT-II complex. Biochem. Biophys. Res. Commun. 2006; 347:1074-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6799

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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