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Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.

RMI1 Antibody

CATALOG NUMBER: 26-127

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RMI1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RMI1 antibody can be used for detection of RMI1 by ELISA at 1:1562500. RMI1 antibody can be used for detection of RMI1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Tranfected 293T Cell Lysate
Predicted Molecular Weight:
70 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store RMI1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
RMI1
Additional Names:
RMI1, BLAP75, C9orf76, FLJ12888, RP11-346I8.1, FAAP75
Protein Accession Number:
NP_079221
Protein Gi Number:
153791761
Ncbi Gene Id Number:
80010
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RMI1 is an essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. RMI1 promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. RMI1 is required for BLM phosphorylation during mitosis. Within the BLM complex, RMI1 is required for BLM and TOP3A stability.RMI1 is a component of protein complexes that limit DNA crossover formation via the dissolution of double Holliday junctions (Raynard et al., 2006 [PubMed 16595695]).
Background Reference 1:
Broberg, K., (2007) Cancer Lett. 258 (1), 38-44.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-127

List Size:
100 ul

List Price:

$519.00

Datasheet
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