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Western blot analysis of extracts of various cell lines, using RNASE12 antibody (15-628) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using RNASE12 antibody (15-628) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

RNASE12 Antibody

CATALOG NUMBER: 15-628

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RNASE12 (NP_001019993.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
22Rv1
Positive Control 2:
293T
Predicted Molecular Weight:
Observed: 17kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RNASE12
Additional Names:
HEL-S-85p, RAI1, probable inactive ribonuclease-like protein 12, epididymis secretory sperm binding protein Li 85p, ribonuclease A I1, ribonuclease, RNase A family, 12 (non-active), ribonuclease-like protein 12
Ncbi Gene Id Number:
493901
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-628

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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