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Western blot analysis of extracts of Raji cells, using RNASE13 antibody (13-774) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human prostate using RNASE13 antibody (13-774) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RNASE13 antibody (13-774) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using RNASE13 antibody (13-774) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RNASE13 antibody (13-774). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of Raji cells, using RNASE13 antibody (13-774) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

RNASE13 Antibody

CATALOG NUMBER: 13-774

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human RNASE13 (NP_001012264.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
Raji
Predicted Molecular Weight:
Observed: 18kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RNASE13
Additional Names:
HEL-S-86p, RAL1, probable inactive ribonuclease-like protein 13, epididymis secretory sperm binding protein Li 86p, ribonuclease 13, ribonuclease A L1, ribonuclease, RNase A family, 13 (non-active), ribonuclease-like protein 13
Ncbi Gene Id Number:
440163
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-774

List Size:
100 uL

List Price:

$436.00

Datasheet
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