Western blot analysis of RNF11 using rabbit polyclonal RNF11 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RNF11 gene.

RNF11 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of RNF11 using rabbit polyclonal RNF11 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RNF11 gene.

RNF11 Antibody

CATALOG NUMBER: 56-726

Clonality:

Polyclonal

Tested Applications:

Flow, IHC-P, WB

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Homology:

Predicted species reactivity based on immunogen sequence: Bovine, Mouse

Immunogen:

This RNF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-90 amino acids from the Central region of human RNF11.

Conjugate:

Unconjugated

Tested Applications:

Flow, IHC-P, WB

Application Note:

For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50

Predicted Molecular Weight:

17 kDa

Advanced Validation

Properties

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Clonality:

Polyclonal

Isotype:

Rabbit Ig

Physical State:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

RNF11

Additional Names:

RING finger protein 11, RNF11

Protein Accession Number:

Q9Y3C5

Protein Gi Number:

21362884

Ncbi Gene Id Number:

26994

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

The protein encoded by this gene contains a RING-H2 fingermotif, which is known to be important for protein-proteininteractions. The expression of this gene has been shown to beinduced by mutant RET proteins (MEN2A/MEN2B). The germlinemutations in RET gene are known to be responsible for thedevelopment of multiple endocrine neoplasia (MEN). [provided byRefSeq].

Background Reference 1:

Santonico, E., et al. Oncogene 29(41):5604-5618(2010)

Background Reference 2:

Markson, G., et al. Genome Res. 19(10):1905-1911(2009)

Background Reference 3:

Shembade, N., et al. EMBO J. 28(5):513-522(2009)

Background Reference 4:

van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-726

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

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RNF11 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Homology:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Protein Gi Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Background Reference 4:

Disclaimer:

List Size:

Shipping Info

Customize your order

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