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46-303 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum/Golgi and some nuclear stain
46-303 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
46-303 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum/Golgi and some nuclear stain

RNF125 Antibody

CATALOG NUMBER: 46-303

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-RSLLEYVNHSNTT
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum/Golgi of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RNF125
Additional Names:
RNF125, FLJ20456, MGC21737, ring finger protein 125, TRAC1, T-cell ring protein identified in activation screen
Protein Accession Number:
NP_060301.2
Protein Gi Number:
37595555
Ncbi Gene Id Number:
54941
Background Reference 1:
Chu P, Pardo J, Zhao H, Li CC, Pali E, Shen MM, Qu K, Yu SX, Huang BC, Yu P, Masuda ES, Molineaux SM, Kolbinger F, Aversa G, De Vries J, Payan DG, Liao XC. Systematic identification of regulatory proteins critical for T-cell activation. J Biol. 2003;2(3):21. Epub 2003 Sep 15.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-303

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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