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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Western blot analysis in HL-60 cell line lysates (35ug/lane).

RNF14 Antibody

CATALOG NUMBER: 59-350

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RNF14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the C-terminal region of human RNF14.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RNF14
Additional Names:
E3 ubiquitin-protein ligase RNF14, 632-, Androgen receptor-associated protein 54, HFB30, RING finger protein 14, Triad2 protein, RNF14, ARA54
Protein Accession Number:
Q9UBS8
Protein Gi Number:
17380293
Ncbi Gene Id Number:
9604
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene contains a RING zincfinger, a motif known to be involved in protein-proteininteractions. This protein interacts with androgen receptor (AR)and may function as a coactivator that induces AR target geneexpression in prostate. A dominant negative mutant of this gene hasbeen demonstrated to inhibit the AR-mediated growth of prostatecancer. This protein also interacts with class IIIubiquitin-conjugating enzymes (E2s) and may act as aubiquitin-ligase (E3) in the ubiquitination of certain nuclearproteins. Five alternatively spliced transcript variants encodingtwo distinct isoforms have been reported.
Background Reference 1:
Xu, K., et al. Cancer Cell 15(4):270-282(2009)
Background Reference 2:
Lan, K.C., et al. Fertil. Steril. 89 (5 SUPPL), 1397-1405 (2008) :
Background Reference 3:
Kikuchi, H., et al. Carcinogenesis 28(8):1752-1758(2007)
Background Reference 4:
Yang, Z., et al. Endocrinology 148(3):1340-1349(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-350

List Size:
400 ul

List Price:

$495.00

Datasheet
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