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Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
RNF144A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).

RNF144A Antibody

CATALOG NUMBER: 57-706

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RNF144A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the Central region of human RNF144A.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RNF144A
Additional Names:
E3 ubiquitin-protein ligase RNF144A, 632-, RING finger protein 144A, UbcM4-interacting protein 4, Ubiquitin-conjugating enzyme 7-interacting protein 4, RNF144A, KIAA0161, RNF144, UBCE7IP4
Protein Accession Number:
P50876
Protein Gi Number:
160358924
Ncbi Gene Id Number:
9781
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this protein contains a RINGfinger, a motif known to be involved in protein-DNA andprotein-protein interactions. The mouse counterpart of this proteinhas been shown to interact with Ube2l3/UbcM4, which is anubiquitin-conjugating enzyme involved in embryonic development.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Adkins, D.E., et al. Mol. Psychiatry (2010) In press :
Background Reference 3:
Hoja, M.R., et al. Exp. Cell Res. 259(1):239-246(2000)
Background Reference 4:
Martinez-Noel, G., et al. FEBS Lett. 454(3):257-261(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-706

List Size:
400 ul

List Price:

$495.00

Datasheet
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