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Western blot analysis of extracts of various cell lines, using RNF2 antibody (19-735) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using RNF2 antibody (19-735) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RNF2 antibody (19-735) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RNF2 antibody (19-735) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug RNF2 antibody (19-735). Western blot was performed from the immunoprecipitate using RNF2 antibody (19-735) at a dilition of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RNF2 antibody (19-735) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of extracts of various cell lines, using RNF2 antibody (19-735) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

RNF2 Antibody

CATALOG NUMBER: 19-735

Clonality:
Polyclonal
Tested Applications:
ChIP, IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RNF2 (NP_009143.1).
Conjugate:
Unconjugated
Tested Applications:
ChIP, IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
ChIP: 1:50 - 1:200
Positive Control 1:
293T
Positive Control 2:
Jurkat
Positive Control 3:
U-937
Positive Control 4:
SH-SY5Y
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse spleen
Predicted Molecular Weight:
Observed: 38kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RNF2
Additional Names:
RNF2, BAP1, DING, BAP-1, HIPI3, RING2, RING1B
Ncbi Gene Id Number:
6045
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-735

List Size:
100 uL

List Price:

$436.00

Datasheet
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