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46-304 staining (0.5 ug/ml) of K562 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
46-304 staining (0.5 ug/ml) of K562 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

RNF2 Antibody

CATALOG NUMBER: 46-304

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-PMELYYAPTKEHK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx. 37-38kDa band observed in lysates of cell lines of NIH3T3 and K562 (calculated MW of 37.7kDa according to NP_009143.1). Recommended concentration: 0.3-1ug/ml.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RNF2
Additional Names:
RNF2, DING, ring finger protein 2, BAP-1, Bap-1, BAP1, HIPI3, RING1B, RING2, GS1-120K12.1
Protein Accession Number:
NP_009143.1
Protein Gi Number:
6005747
Ncbi Gene Id Number:
6045
Background Reference 1:
Tuckfield A, Clouston DR, Wilanowski TM, Zhao LL, Cunningham JM, Jane SM Binding of the RING polycomb proteins to specific target genes in complex with the grainyhead-like family of developmental transcription factors. Mol Cell Biol 2002 Mar;22(6):1936-46

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-304

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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