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Antibody used in IHC on Human Skin.
Antibody used in WB on Human HepG2 at 0.5 ug/ml.
Antibody used in IHC on Human Skin.

RNF25 Antibody

CATALOG NUMBER: 29-853

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF25.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
RNF25 antibody can be used for detection of RNF25 by ELISA at 1:62500. RNF25 antibody can be used for detection of RNF25 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
51 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store RNF25 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
RNF25
Additional Names:
RNF25, AO7, FLJ13906
Protein Accession Number:
NP_071898
Protein Gi Number:
34878787
Ncbi Gene Id Number:
64320
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RNF25 contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.
Background Reference 1:
Asamitsu, K., (2003) J. Biol. Chem. 278 (29), 26879-26887.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-853

List Size:
100 ul

List Price:

$519.00

Datasheet
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