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Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in CEM cell line lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

RNF31 Antibody

CATALOG NUMBER: 60-435

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RNF31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 955-983 amino acids from the C-terminal region of human RNF31.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
120 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RNF31
Additional Names:
E3 ubiquitin-protein ligase RNF31, 632-, HOIL-1-interacting protein, HOIP, RING finger protein 31, Zinc in-between-RING-finger ubiquitin-associated domain protein, RNF31, ZIBRA
Protein Accession Number:
Q96EP0
Protein Gi Number:
45477216
Ncbi Gene Id Number:
55072
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.
Background Reference 1:
Silva, L.K., et al. Eur. J. Hum. Genet. (2010) In pressEhrlund, A., et al. Mol. Cell. Biol. 29(8):2230-2242(2009) Tokunaga, F., et al. Nat. Cell Biol. 11(2):123-132(2009) Kirisako, T., et al. EMBO J. 25(20):4877-4887(2006) Lim, J., et al. Cell 125(4):801-814(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-435

List Size:
400 ul

List Price:

$495.00

Datasheet
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