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46-311 (0.5ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-311 (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-311 (0.5ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RNF36 Antibody

CATALOG NUMBER: 46-311

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-GENKEPLHILHPQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Mouse Testis lysates (calculated MW of 57.3kDa according to Mouse NP_536771.2). Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:In paraffin embedded Human Testis shows speckled staining in nuclei of spermatocytes and spermitids. Recommended concentration, 2.5-3.8ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_892030.3; NP_542783.2).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TRIM69
Additional Names:
TRIM69 , tripartite motif-containing 69, HSD34, RNF36, Trif, ring finger protein 36, trimless, tripartite motif protein 69, hCG_39321, RNF36
Protein Accession Number:
NP_892030.3,NP_542783.2
Protein Gi Number:
88999601
Ncbi Gene Id Number:
140691
Background Reference 1:
Shyu HW, Hsu SH, Hsieh-Li HM, Li H. A novel member of the RBCC family, Trif, expressed specifically in the spermatids of mouse testis. Mech Dev 2001 Oct;108(1-2):213-6

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-311

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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