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Western Blot at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-T in K562 cell line lysates (35ug/lane).
Western Blot at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.

RNF36 Antibody

CATALOG NUMBER: 61-414

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RNF36 (TRIM69) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-231 amino acids from the Central region of human RNF36 (TRIM69).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIM69
Additional Names:
E3 ubiquitin-protein ligase TRIM69, 632-, RFP-like domain-containing protein trimless, RING finger protein 36, Tripartite motif-containing protein 69, TRIM69, RNF36
Protein Accession Number:
Q86WT6
Protein Gi Number:
229462760
Ncbi Gene Id Number:
140691
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRIM69 is a member of the RING-B-box-coiled-coil (RBCC) family with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of TRIM69 is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis.
Background Reference 1:
Shyu,H.W., Exp. Cell Res. 287 (2), 301-313 (2003)
Background Reference 2:
Shyu,H.W., Mech. Dev. 108 (1-2), 213-216 (2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-414

List Size:
400 ul

List Price:

$495.00

Datasheet
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