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Western blot analysis of extracts of various cell lines, using RNF4 antibody (14-233) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using RNF4 antibody (14-233) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

RNF4 Antibody

CATALOG NUMBER: 14-233

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RNF4 (NP_001171938.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:1000
Positive Control 1:
Mouse kidney
Positive Control 2:
Mouse brain
Positive Control 3:
Rat testis
Predicted Molecular Weight:
Observed: 21kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RNF4
Additional Names:
RES4-26, SLX5, SNURF, E3 ubiquitin-protein ligase RNF4, E3 ubiquitin ligase RNF4, RING-type E3 ubiquitin transferase RNF4, small nuclear RING finger protein
Ncbi Gene Id Number:
6047
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-233

List Size:
100 uL

List Price:

$436.00

Datasheet
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