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Western blot analysis of extracts of various cell lines, using RNF40 antibody (22-226) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using RNF40 antibody (22-226).
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RNF40 antibody (22-226) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of extracts of various cell lines, using RNF40 antibody (22-226) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

RNF40 Antibody

CATALOG NUMBER: 22-226

Clonality:
Polyclonal
Tested Applications:
ChIP, IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RNF40 (NP_055586.1).
Conjugate:
Unconjugated
Tested Applications:
ChIP, IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:100
ChIP: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
HepG2
Positive Control 3:
U-937
Predicted Molecular Weight:
Observed: 140kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RNF40
Additional Names:
RNF40, BRE1B, DKFZp686K191, KIAA0661, MGC13051, RBP95, STARING
Ncbi Gene Id Number:
9810
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-226

List Size:
100 uL

List Price:

$436.00

Datasheet
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