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Western blot analysis of RNF40 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate.
Western blot analysis of RNF40 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate.

RNF40 Antibody

CATALOG NUMBER: 57-676

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This RNF40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 697-725 amino acids from the C-terminal region of human RNF40.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
114 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RNF40
Additional Names:
E3 ubiquitin-protein ligase BRE1B, BRE1-B, 632-, 95 kDa retinoblastoma-associated protein, RBP95, RING finger protein 40, RNF40, BRE1B, KIAA0661
Protein Accession Number:
O75150
Ncbi Gene Id Number:
9810
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene contains a RING finger, amotif known to be involved in protein-protein and protein-DNAinteractions. This protein was reported to interact with the tumorsuppressor protein RB1. Studies of the rat counterpart suggestedthat this protein may function as an E3 ubiquitin-protein ligase,and facilitate the ubiquitination and degradation of syntaxin 1,which is an essential component of the neurotransmitter releasemachinery.
Background Reference 1:
Chernikova, S.B., et al. Radiat. Res. 174(5):558-565(2010)
Background Reference 2:
Kim, J., et al. Cell 137(3):459-471(2009)
Background Reference 3:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 4:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-676

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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