Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western Blot at 1:2000 dilution + A549 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in CEM cell line lysates (35ug/lane).
RNF43 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:2000 dilution + A549 whole cell lysate Lysates/proteins at 20 ug per lane.

RNF43 Antibody

CATALOG NUMBER: 56-920

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RNF43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543-572 amino acids from the C-terminal region of human RNF43.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
86 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RNF43
Additional Names:
E3 ubiquitin-protein ligase RNF43, 632-, RING finger protein 43, RNF43
Protein Accession Number:
Q68DV7
Protein Gi Number:
74757361
Ncbi Gene Id Number:
54894
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RNF43 is a HAP95 (AKAP8L; MIM 609475) binding ubiquitinligase that promotes cell growth and is upregulated in colon cancer(Yagyu et al., 2004 [PubMed 15492824]; Sugiura et al., 2008 [PubMed18313049]).
Background Reference 1:
Low, S.K., et al. PLoS ONE(7), E11824 (2010) :
Background Reference 2:
Sugiura, T., et al. Exp. Cell Res. 314(7):1519-1528(2008)
Background Reference 3:
Uchida, N., et al. Clin. Cancer Res. 10(24):8577-8586(2004)
Background Reference 4:
Yagyu, R., et al. Int. J. Oncol. 25(5):1343-1348(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-920

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369