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Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.

RNF5 Antibody

CATALOG NUMBER: 27-144

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RNF5 antibody can be used for detection of RNF5 by ELISA at 1:12500. RNF5 antibody can be used for detection of RNF5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
20 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store RNF5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
RNF5
Additional Names:
RNF5, RING5, RMA1
Protein Accession Number:
NP_008844
Protein Gi Number:
5902054
Ncbi Gene Id Number:
6048
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RNF5 contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background Reference 1:
Bromberg, K.D., (2007) Cancer Res. 67 (17), 8172-8179.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-144

List Size:
100 ul

List Price:

$499.00

Out of stock

Datasheet
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