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42-275 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-275 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-275 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RNF7 Antibody

CATALOG NUMBER: 42-275

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: DACLRCQAENKQE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 11kDa band observed in Human Human Heart lysates (calculated MW of 12.7kDa according to NP_055060.1). Recommended concentration: 1-3ug/ml.An additional band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 (NP_055060.1).
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RNF7
Additional Names:
RNF7, ring finger protein 7, CKBBP1, ROC2, SAG, OTTHUMP00000180645, regulator of cullins 2, sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2, zinc RING finger protein SAG
Protein Accession Number:
NP_055060.1
Protein Gi Number:
7657522
Ncbi Gene Id Number:
9616
Background Reference 1:
Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA, E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Molecular cell 2009 Feb 33 (4): 483-95.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-275

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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