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46-313 (1ug/ml) staining of HepG2 (A) and Jurkat (B) nuclear cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-313 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
46-313 (1ug/ml) staining of HepG2 (A) and Jurkat (B) nuclear cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.

RNF8 Antibody

CATALOG NUMBER: 46-313

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: GEPGFFVTGDRAG-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Testis and Lung lysates and approx. 50kDa in nuclear lysates of cell lines HepG2 and Jurkat (predicted MW of 56kDa according to NP_003949.1 and 50.8kDa according to NP_898901.1 ). Recommended concentration 0.5-2ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1).
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RNF8
Additional Names:
RNF8, ring finger protein 8, ring finger protein (C3HC4 type) 8, KIAA0646, FLJ12013, C3HC4-type zinc finger protein, OTTHUMP00000039684, UBC13/UEV-interacting ring finger protein,
Protein Accession Number:
NP_003949.1,NP_898901.1
Protein Gi Number:
4504867
Ncbi Gene Id Number:
9025
Background Reference 1:
Seki N, Hattori A, Sugano S, Suzuki Y, Nakagawara A, Ohhira M, Muramatsu M, Hori T, Saito T. Isolation, tissue expression, and chromosomal assignment of a novel human gene which encodes a protein with RING finger motif. J Hum Genet. 1998;43(4):272-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-313

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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