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Western blot analysis of lysate from HT29 cell line,using RNH1 Antibody at 1:1000 at each lane
Confocal immunofluorescent analysis of RNH1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of lysate from HT29 cell line,using RNH1 Antibody at 1:1000 at each lane

RNH1 Antibody

CATALOG NUMBER: 56-428

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RNH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-454 amino acids from the C-terminal region of human RNH1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RNH1
Additional Names:
Ribonuclease inhibitor, Placental ribonuclease inhibitor, Placental RNase inhibitor, Ribonuclease/angiogenin inhibitor 1, RAI, RNH1, PRI, RNH
Protein Accession Number:
P13489
Protein Gi Number:
132573
Ncbi Gene Id Number:
6050
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Placental ribonuclease inhibitor (PRI) is a member of afamily of proteinaceous cytoplasmic RNase inhibitors that occur inmany tissues and bind to both intracellular and extracellularRNases (summarized by Lee et al., 1988 [PubMed 3219362]). Inaddition to control of intracellular RNases, the inhibitor may havea role in the regulation of angiogenin (MIM 105850). Ribonucleaseinhibitor, of 50,000 Da, binds to ribonucleases and holds them in alatent form. Since neutral and alkaline ribonucleases probably playa critical role in the turnover of RNA in eukaryotic cells, RNH maybe essential for control of mRNA turnover; the interaction ofeukaryotic cells with ribonuclease may be reversible invivo.
Background Reference 1:
Martins-de-Souza, D., et al.Psychiatr Res 44(14):989-991(2010)
Background Reference 2:
Martins-de-Souza, D., et al.Neural Transm 116(3):275-289(2009)
Background Reference 3:
Turcotte, R.F., et al. Biochem. Biophys. Res. Commun. 377(2):512-514(2008)
Background Reference 4:
Johnson, R.J., et al. Biochemistry 46(45):13131-13140(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-428

List Size:
400 ul

List Price:

$495.00

Datasheet
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