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46-315 (1ug/ml) staining of HUVEC lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-315 (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-315 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi and membrane staining. The nuclear stain is
46-315 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black lin
46-315 (1ug/ml) staining of HUVEC lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Robo1 Antibody

CATALOG NUMBER: 46-315

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-GDVDLSNKINEMK
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:An anonymous source observed an approx 250kDa band in Human Endothelial cell lysates at 1ug/ml antibody concentration, thought to be due to glycosylation. Some proteolytic breakdown products were also seen. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 2ug/ml.Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Specificity:
Not expected to cross-react with ROBO2 or ROBO3.
Positive Control 1:
HUVEC Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ROBO1
Additional Names:
ROBO1, DUTT1, roundabout, axon guidance receptor, homolog 1 (Drosophila) , HGNC:10249, FLJ21882, SAX3, axon guidance receptor, roundabout 1, MGC131599, MGC133277
Protein Accession Number:
NP_002932.1,NP_598334.1
Protein Gi Number:
4506569
Ncbi Gene Id Number:
6091
Background Reference 1:
Hannula-Jouppi K, Kaminen-Ahola N, Taipale M, Eklund R, Nopola-Hemmi J, Kaariainen H, Kere J. The Axon Guidance Receptor Gene ROBO1 Is a Candidate Gene for Developmental Dyslexia. PLoS Genet. 2005 Oct;1(4):e50. Epub 2005 Oct 28.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-315

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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