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ROBO3 Antibody

CATALOG NUMBER: 42-734

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-RKDGARLKEEEGR
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Western Blot: Preliminary experiments gave an approx 25kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 148kDa according to NP_071765.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Flow cytometry: This antibody was successfully used on rheumatoid arthritis synovial fibroblasts (RASF) as described in Denk et al, Arthritis Res Ther. 2010;12(2):R45.; PMID: 20298552.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ROBO3
Additional Names:
ROBO3, roundabout, axon guidance receptor, homolog 3 (Drosophila) , HGNC:13433, FLJ21044, HGPPS, RBIG1, RIG1 , retinoblastoma inhibiting gene 1, roundabout, axon guidance receptor, homolog 3
Protein Accession Number:
NP_071765.2
Protein Gi Number:
48476182
Ncbi Gene Id Number:
64221

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-734

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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