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Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of ROCK1 in 293 cells with ROCK1 antibody at 10 μg/mL.
Immunofluorescence of ROCK1 in 293 cells with ROCK1 antibody at 20 μg/mL.
Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ROCK1 Antibody

CATALOG NUMBER: 6295

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (93%)
Immunogen:
ROCK1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1.
The immunogen is located within the first 50 amino acids of ROCK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
ROCK1 antibody can be used for detection of ROCK1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
ROCK1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ROCK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ROCK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ROCK1
Additional Names:
ROCK1 Antibody: ROCK-I, P160ROCK, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, ROCK-I
Protein Accession Number:
NP_005397
Protein Gi Number:
4885583
Ncbi Gene Id Number:
6093
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ROCK1 Antibody: ROCK1 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK1 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK1, like its isozyme ROCK2, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.
Background Reference 1:
Ishizaki T, Maekawa M, Fujisawa K, et al. The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase. EMBO J. 1996; 15:1885-93.
Background Reference 2:
Takahashi N, Tuiki H, Saya H, et al. Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24. Genomics 1999; 55:235-7.
Background Reference 3:
Leung T, Chen XQ, Manser E, et al. The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton. Mol. Cell Biol. 1996; 16:5313-27.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6295

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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