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Western blot analysis in mouse testis tissue lysates (35ug/lane).
ROPN1L Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse testis tissue lysates (35ug/lane).

ROPN1L Antibody

CATALOG NUMBER: 56-072

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey
Immunogen:
This ROPN1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-60 amino acids from the N-terminal region of human ROPN1L.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
26 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ROPN1L
Additional Names:
Ropporin-1-like protein, ROPN1-like protein, AKAP-associated sperm protein, ROPN1L, ASP
Protein Accession Number:
Q96C74
Protein Gi Number:
296452880
Ncbi Gene Id Number:
83853
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a sperm protein, whichinteracts with A-kinase anchoring protein, AKAP3, through theamphipathic helix region of AKAP3. Type II regulatory subunit ofcAMP-dependent protein kinase (PKARII) also binds to this helixdomain of AKAP3, which allows PKARII to be targeted to specificsubcellular compartments. It is suggested that sperm containsseveral proteins that bind to AKAPs in a manner similar to PKARII,and this encoded protein may be one of them.
Background Reference 1:
Carr, D.W., et al. J. Biol. Chem. 276(20):17332-17338(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-072

List Size:
400 ul

List Price:

$495.00

Datasheet
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