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Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Antibody staining ROR1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining ROR1 in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Immunohistochemical analysis of paraffin-embedded H. heart section using ROR1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysates from K562 cell line, human lung, mouse kidney, mouse heart tissue (from left to right), using ROR1 Antibody at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the ROR1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

ROR1 Antibody

CATALOG NUMBER: 63-129

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ROR1 antibody is generated from rabbits immunized with recombinant human ROR1 protein (aa region: 112 - 399).
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For FACS starting dilution is: 1:25
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
104 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ROR1
Additional Names:
Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase, receptor-related 1, ROR1, NTRKR1
Protein Accession Number:
Q01973
Protein Gi Number:
118572711
Ncbi Gene Id Number:
4919
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.
Background Reference 1:
Nomi, M., et al., Mol. Cell. Biol. 21(24):8329-8335 (2001).
Background Reference 2:
Reddy, U.R., et al., Genomics 41(2):283-285 (1997).
Background Reference 3:
Reddy, U.R., et al., Oncogene 13(7):1555-1559 (1996).
Background Reference 4:
Masiakowski, P., et al., J. Biol. Chem. 267(36):26181-26190 (1992).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-129

List Size:
200 ul

List Price:

$519.00

Datasheet
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