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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

RORA Antibody

CATALOG NUMBER: 30-078

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RORA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RORA antibody can be used for detection of RORA by ELISA at 1:312500. RORA antibody can be used for detection of RORA by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
62 kDa, 63 kDa, 59 kDa, 54 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store RORA antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
RORA
Additional Names:
RORA, MGC119326, MGC119329, NR1F1, ROR1, ROR2, ROR3, RZRA, RZR-ALPHA
Protein Accession Number:
NP_002934
Protein Gi Number:
4506577
Ncbi Gene Id Number:
6095
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by RORA is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene.The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Four transcript variants encoding different isoforms have been described for this gene.
Background Reference 1:
Boukhtouche, F., (2006) J. Neurochem. 96 (6), 1778-1789.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-078

List Size:
100 ul

List Price:

$473.00

Datasheet
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