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Western blot analysis of anti-RORA Antibody (T216) in A2058 cell line lysates (35ug/lane)
Fluorescent confocal image of Hela cell stained with RORA Antibody (T216).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RORA primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RORA immunoreactivity is localized to Nucleus significantly.
Western blot analysis of anti-RORA Antibody (T216) in A2058 cell line lysates (35ug/lane)

RORA Antibody

CATALOG NUMBER: 55-471

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RORA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from human RORA.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RORA
Additional Names:
Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, Nuclear receptor subfamily 1 group F member 1, RAR-related orphan receptor A, Retinoid-related orphan receptor-alpha, RORA, NR1F1, RZRA
Protein Accession Number:
P35398
Protein Gi Number:
548814
Ncbi Gene Id Number:
6095
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RORA is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene.
Background Reference 1:
Lechtken,A., Biochem. Biophys. Res. Commun. 358 (3), 890-896 (2007)
Background Reference 2:
Lanoix,D.,Hum. Reprod. 21 (8), 1981-1989 (2006)
Background Reference 3:
Boukhtouche,F., J. Neurochem. 96 (6), 1778-1789 (2006)
Background Reference 4:
Klar,J., Eur. J. Hum. Genet. 13 (8), 928-934 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-471

List Size:
400 ul

List Price:

$495.00

Datasheet
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