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Western blot analysis in 293,K562,HL-60 cell line lysates (35ug/lane).
Roundabout 4 Antibody IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Roundabout 4 Antibody for immunohistochemistry.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in 293,K562,HL-60 cell line lysates (35ug/lane).

Roundabout 4 Antibody

CATALOG NUMBER: 61-791

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Roundabout 4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the N-terminal region of human Roundabout 4.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
107 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ROBO4
Additional Names:
Roundabout homolog 4, Magic roundabout, ROBO4
Protein Accession Number:
Q8WZ75
Protein Gi Number:
49036490
Ncbi Gene Id Number:
54538
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Roundabout 4 is receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. This protein may mediate the inhibition of primary endothelial cell migration by Slit proteins.
Background Reference 1:
Jones, C.A., et al. Nat. Cell Biol. 11(11):1325-1331(2009)
Background Reference 2:
Sheldon, H., et al. FASEB J. 23(2):513-522(2009)
Background Reference 3:
Huang, L., et al. Mol. Vis. 15, 1057-1069 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-791

List Size:
400 ul

List Price:

$495.00

Datasheet
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