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Western blot analysis of RP105 in human spleen tissue lysate with RP105 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of RP105 in human spleen tissue with RP105 antibody at 2 μg/mL.
Immunofluorescence of RP105 in human spleen tissue with RP105 antibody at 10 μg/mL.
Western blot analysis of RP105 in human spleen tissue lysate with RP105 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

RP105 Antibody

CATALOG NUMBER: 3845

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
RP105 antibody was raised against a 14 amino acid synthetic peptide from near the center of human RP105.
The immunogen is located within amino acids 170 - 220 of RP105.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RP105 antibody can be used for the detection of RP105 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, RP105 often migrates at 95 - 105 kDa. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
RP105 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RP105 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RP105 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD180
Additional Names:
RP105 Antibody: LY64, Ly78, RP105, LY64, CD180 antigen, Lymphocyte antigen 64
Protein Accession Number:
BAA12019
Protein Gi Number:
1843411
Ncbi Gene Id Number:
4064
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RP105 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The signaling of these TLRs is kept under tight control by the expression of endogenous inhibiting proteins. One such protein is RP105, a recently identified homolog to TLR4 that, with MD-1, interacts with and inhibits the TLR4/MD-2 signaling pathway. It has also been suggested that the RP105/MD-1 complex influences antibody production mediated by both TLR4/MD-2 and TLR2 receptor complexes.
Background Reference 1:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Background Reference 2:
Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
Background Reference 3:
Divanovic S, Trompette A, Atabani SF, et al. Inhibition of TLR-4/MD-2 signaling by RP105/MD-1. J. Endotoxin Res. 2005; 11:363-8.
Background Reference 4:
Nagai Y, Kobayashi T, Motoi Y, et al. The radioprotective 105/MD-1 complex links TLR2 and TLR4/MD-2 in antibody response to microbial membranes. J. Immunol. 2005; 174:7043-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3845

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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