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46-320 staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
46-320 (2.5ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-320 staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

RP6-213H19 Antibody

CATALOG NUMBER: 46-320

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: KLIEKFQKCSADESP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in lysates of Jurkat cell lines (calculated MW of 46.5kDa according to NP_057626.2). Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 2.5ug/ml.
Specificity:
This antibody is expected to recognize all three reported isoforms (NP_057626.2, NP_001035918.1 and NP_001035917.1).
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RP6-213H19.1
Additional Names:
RP6-213H19.1, serine/threonine protein kinase MST4, MASK, MST4, Mst3 and SOK1-related kinase, STE20-like kinase MST4, mammalian Ste20-like protein kinase 4, mammalian sterile 20-like 4, serine/threonine protein kinase MASK
Protein Accession Number:
NP_057626.2,NP_001035918.1,NP_001035917.1
Protein Gi Number:
15011880
Ncbi Gene Id Number:
51765
Background Reference 1:
Dan I, Ong SE, Watanabe NM, Blagoev B, Nielsen MM, Kajikawa E, Kristiansen TZ, Mann M, Pandey A. Cloning of MASK, a novel member of the mammalian germinal center kinase III subfamily, with apoptosis-inducing properties. J Biol Chem. 2002 Feb 22;277(8):5929-39.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-320

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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