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Western blot analysis of extracts of various cell lines, using RPA1 antibody (13-357) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of MCF-7 cells using RPA1 antibody (13-357). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using RPA1 antibody (13-357) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

RPA1 Antibody

CATALOG NUMBER: 13-357

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 367-616 of human RPA1 (NP_002936.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IP: 1:50 - 1:100
Positive Control 1:
U-87MG
Positive Control 2:
Mouse spleen
Predicted Molecular Weight:
Observed: 68kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RPA1
Additional Names:
RPA1, replication protein A1, 70kDa, HSSB, MST075, REPA1, RF-A, RP-A, RPA70, MSTP075, RF-A protein 1, RP-A p70, replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, single-stranded DNA-binding protein
Ncbi Gene Id Number:
6117
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-357

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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