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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).

RPA1 Antibody

CATALOG NUMBER: 57-718

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RPA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-559 amino acids from the C-terminal region of human RPA1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPA1
Additional Names:
Replication protein A 70 kDa DNA-binding subunit, RP-A p70, Replication factor A protein 1, RF-A protein 1, Single-stranded DNA-binding protein, Replication protein A 70 kDa DNA-binding subunit, N-terminally processed, RPA1, REPA1, RPA70
Protein Accession Number:
P27694
Protein Gi Number:
1350579
Ncbi Gene Id Number:
6117
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RPA1 plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Background Reference 1:
Choi, J.H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(31):13660-13665(2010)
Background Reference 2:
Locatelli, G.A., et al. Biochem. J. 429(3):573-582(2010)
Background Reference 3:
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Background Reference 4:
Guillem, V.M., et al. Am. J. Hematol. 85(7):482-486(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-718

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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