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Western blot analysis of RPA43 Antibody in Jurkat,K562 cell line lysates (35ug/lane)
RPA43 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of RPA43 Antibody in Jurkat,K562 cell line lysates (35ug/lane)

RPA43 Antibody

CATALOG NUMBER: 61-860

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RPA43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-326 amino acids from the C-terminal region of human RPA43.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TWISTNB
Additional Names:
DNA-directed RNA polymerase I subunit RPA43, Twist neighbor protein, TWISTNB
Protein Accession Number:
Q3B726
Protein Gi Number:
121942339
Ncbi Gene Id Number:
221830
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RPA43 is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters.
Background Reference 1:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 2:
Yuan, X., et al. EMBO Rep. 3(11):1082-1087(2002)
Background Reference 3:
Kosan, C., et al. Cytogenet. Genome Res. 97 (3-4), 167-170 (2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-860

List Size:
400 ul

List Price:

$495.00

Datasheet
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