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46-321 (0.1ug/ml) staining of Rat Retina lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-321 (0.1ug/ml) staining of Rat Retina lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

RPE65 Antibody

CATALOG NUMBER: 46-321

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog
Immunogen:
The immunogen for this antibody is: C-EVKKNARKAPQPE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Rat Retina lysates (calculated MW of 60.9kDa according to Human NP_000320.1 and 61.0kDa according to Rat NP_446014.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.
Positive Control 1:
Rat Retina Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RPE65
Additional Names:
mRPE65, sRPE65, retinal pigment epithelium-specific protein 65kDa, LCA2, RP20, rd12, RBP-binding membrane protein, p63, retinal pigment epithelium-specific 61 kDa protein, retinal pigment epithelium-specific protein (65kD), retinitis pigmentosa 20
Protein Accession Number:
NP_000320.1
Protein Gi Number:
4506591
Ncbi Gene Id Number:
6121
Background Reference 1:
Rolling F, Le Meur G, Stieger K, Smith AJ, Weber M, Deschamps JY, Nivard D, Mendes-Madeira A, Provost N, Pereon Y, Cherel Y, Ali RR, Hamel C, Moullier P, Rolling F. Gene therapeutic prospects in early onset of severe retinal dystrophy: restoration of vision in RPE65 Briard dogs using an AAV serotype 4 vector that specifically targets the retinal pigmented epithelium. Bull Mem Acad R Med Belg. 2006;161(10-12):497-508; discussion 508-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-321

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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